Implemented kill switches: 1
Kill Switch 1
Class
# Format: # Write how you would classify them. Prevention of horizontal gene transfer through the inhibition of a plasmid-expressed toxin by genomically integrated antitoxin
Toxins
| Name | Number | Comment |
|---|---|---|
| J23103 promoter - RBS B0034-RFP E1010 - Holin K112805 - endolysin K112806 | BBa_K515106 | Cassette for constitutive expression of holin and endolysin |
Antagonists
| Name | Number | Comment |
|---|---|---|
| J23100 promoter - Antiholin | BBa_K515104 | Antiholin with insulator and RBS and double TAA terminator for genomic integration |
Topology
Parts
| Name | Number | Comment |
|---|---|---|
| J23103 promoter - RBS B0034-RFP E1010 - Holin K112805 - endolysin K112806 | BBa_K515106 | Cassette for constitutive expression of holin and endolysin |
| J23100 promoter - Antiholin | BBa_K515004 | Antiholin with insulator and RBS and double TAA terminator for genomic integration and constitutive expression |